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"I AM A WOMAN OF GOD" WOMAN'S CONFERENCE Group

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Chandrasen Pena
Chandrasen Pena

112115


Hi, Regarding the ab112115 kit, I would like to know if you have any idea if fetal bovine serum might interfere in the measurement of the calcium in the sample solution, since some proteins from the serum can form complexes with calcium. Thanks. Regards,




112115



Hi, I purchased the ab112115 kit. I would like to know if it is possible to freeze the Assay Reaction Mixture (when the Red stock solution and the assay buffer are mixed) once we have prepare it, in aliquots, and for how long can this be preserved. In the protocol it is only mentioned the possibility of freezing the mix of water with the red stock solution. Thanks,


Hi!I'm interested in the product: ab112115. My intention is to use it to measure a gradient of Ca+2 by fluorescent microscopy and study cell chimotaxis at the same time. I would like to know if this product can work in this conditions: physiological conditions of pH and Tº, and if it might be toxic for the cells.Last but not least, I would like to know what proportion of probe is recommended to use in front of a specific concentration of calcium.I will deeply appreciate your help.


Thank you for contacting us and your interest in our products.The Calcium Quantification Assay kit (Fluorometric), ab112115, is intended for use with solution based samples. For your experiment, it may be better to have a look at the Fluo-8 Medium Removal Calcium Assay kit (ab112128) or the Fluo-8 No Wash Calcium Assay kit (ab112129) to see if you think these would be suitable for you.I hope this information has been of help. If I can be of any further assistance, please do not hesitate to contact us again.


Hi, I'm interested in using this Calcium fluorometer Ref.112115 in order to measure calcium gradient in medium and study cell chimotaxis. I'd like to know for how long does this product stay stable once you add it in the sample, I mean, how long can you wait to measure fluorescence once you introduce it in the sample. Thanks a lot!


Thank you for contacting us yesterday in regards to the Calcium Quantification Assay kit (Fluorometric), ab112115. I now have some further information to share with you in regards to the kit. Unfortunately, I can confirm that the kit is likely to cross-react with zinc (as well as cadmium, mercury, lead and manganese). I am sorry but we do not currently have any kits available from our catalogue where this is not going to be a problem for you (I am confirming this with one final kit and I will get back to you with this information as soon as I have it). I can however say that the protein in your aqueous solution should not significantly affect the assay. As discussed over the phone, this kit can measure down to 0.03mM. If you are interested in detecting levels much below this I would suggest having a look at the Fluo-8 No Wash Calcium Assay kit (ab112129). This kit is able to measure much lower calcium concentrations (however, this kit will also pick up zinc). I hope this information has been of some help. If you have any further questions, please do not hesitate to contact us again. 041b061a72


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